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. 2023 May 5;220(8):e20221166. doi: 10.1084/jem.20221166

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© 2023 Yang et al.

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Figure S5. — Clinical candidate rhMVA induces innate immunity and promotes maturation of human moDCs. (A) Schematic diagram for the generation of rhMVA through homologous recombination. (B) Representative flow cytometry plots of expression of hFlt3L or hOX40L by rMVA-infected B16-F10 cells and SK-MEL-28 cells. (C) Relative mRNA expression levels of ifnb, ccl4, ccl5, cxcl10, il1b, il6, and tnf in moDCs infected with MVA or rhMVA. A representative experiment is shown, repeated once. (D) Mean fluorescence intensity of CD86 expressed by human moDCs infected with MVA or rhMVA. A representative experiment is shown, repeated once.