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Real-time imaging of the synchronized trafficking of HLA-E-SBP-EGFP. HeLa cells were transfected to express sec61B-streptavidin as a hook and HLA-E_SBP_EGFP (green) as a reporter. 24 h after transfection, biotin (final 50 μM) was added to release HLA-E, and its trafficking was monitored by acquiring images every minute for up to 2 h using a confocal microscope. Video shown here is representative of two independent experiments. Frame rate, 4 frames/min. Scale bar = 20 μm.
