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Effects of the protease treatment of unprocessed iota-toxin components on the cytopathic activity. Unp-Ib (10−7 M) was treated with protease for 20 min at room temperature, blocked with soybean trypsin inhibitor and Pefablock, and then combined with Pr-Ia (10−7 M). Unp-Ia was treated under the same conditions and then combined with Pr-Ib. The Vero cell rounding was recorded after 21 h of incubation. Means and standard deviations are shown (n = 4).
