FIG. 3.

Mean IL-2 (A) and IFN-γ (B) levels in spleen cell culture supernatant from BALB/c and B10 mice vaccinated with plasmid DNA encoding Ag85A by gg (2 or 10 μg) or i.m. (10 or 100 μg) and restimulated in vitro with purified Ag85A protein. IL-2 was measured in a 24-h culture supernatant using a IL-2-dependent CTLL-2 line. Results are expressed as the mean cpm ± the SD from four individual mice/group. IFN-γ was measured in a 72-h culture supernatant using ELISA. Data are expressed as the mean picograms/milliliter ± the SD from four individual mice/group.