Table 1.
Genes targeted for each virus and each assay.
| SARS-CoV-2 | Influenza A | Influenza B | RSV | |
|---|---|---|---|---|
| SOC | E; RdRpa | M | M | M for RSVA/RSVBb |
| FAO | – | M | – | – |
| GXc | E; N2; RdRp | M; PA; PB2 | M; NSP | N for RSVA/RSVB |
| TPd | N; S | M | M | N for RSVA, M for RSVB |
| PCc | RdRp | M | NP | N for RSVA/RSVB |
SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; IAV, Influenza A virus; IBV, Influenza B virus; RSVA/B, respiratory syncytial virus A/B; SoC, standard of care; FAO, Food and Agriculture Organization of United Nations; GX, Xpert® Xpress SARS-CoV-2, Flu, RSV Kit; TP, TaqPath™ COVID-19, FluA/B, RSV Combo Kit; PC, PowerChek™ SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit II; E, Envelop; M, Matrix; N, nucleocapsid; NP, nucleoprotein; NSP, non-structural protein; PA, polymerase acidic protein; PB2, polymerase basic protein; RdRp, RNA-dependent RNA polymerase; S, spike. MPAs target one, two or three genes for detection of each SARS-CoV-2, IAV, IBV and RSV.
SoC use two sets of primers and probes performed in two separate wells/PCR runs for each sample for detection of SARS-CoV-2.
SoC use different optical channels to detect the RSV targets and then provide separate results for RSVA and RSVB.
GX and PC use separate optical channels to detect SARS-CoV-2, IAV, IBV and RSV and provide results for each virus separately.
TP uses one optical detection channel for the detection of IAV and IBV and provides a combined result for influenza A/B, and similarly one optical channel is used for detection of RSVA and RSVB providing a combined result for RSV.