Table 2.

Comparison of limit of detection between evaluated and standard assays.

Virus	Host	Subtype	Lineage	FAO	GX	TP	PC
SARS-CoV-2	Human	Wuhan	Indian, B.6, 2000	Not done	0	0	0
		Alpha	2021	Not done	−1	1	1
		Omicron	BA.2, 2022	Not done	0	0	0
Influenza	Human seasonal influenza	A/H1N1	pdm, 2019	−2	−1	1	1
		A/H3N2	2019	1	0	0	1
		A/H3N2	2022	0	−1	0	0
		B/Vic	Victoria	Not done	1	0	1
		B/Yam	Yamagata	Not done	1	2	1
	Avian influenza in human cases	A/H5N1	2.3.2.1c 2014	0	2	1	1
		A/H9N2	G9/BJ94 2021	1	0	1	2
	Avian influenza in poultry samples	A/H5N1	2.3.2.1c 2021	0	0	0	1
		A/H5N8	2.3.4.4b 2022	0	0	0	0
		A/H7N4	Jiangsu 2018	0	−1	−1	0

FAO, Food and Agriculture Organization of United Nations recommended primers and probes developed by the Australian Center for Disease Prevention for the detection of M gene from avian influenza; GX, Xpert^® Xpress SARS-CoV-2, Flu, RSV Kit; TP, TaqPath™ COVID-19, FluA/B, RSV Combo Kit; PC PowerChek™ SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit II; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. The table presents the difference between SoC and evaluated assay in term of Log10 dilution. Delta LoD resulted in 0 if MPA and SoC had the same LoD (in light green), ≥1 if LoD of MPA was lower than SoC (in dark green, bold) and <0 if LoD of MPA was higher than SoC (in red). Delta LoD resulted in 0 if MPA and SoC had the same LoD, and <0 if LoD of MPA was higher than SoC.