Table 3.
Comparison of evaluated assay and standard WHO/GIRS assays currently used in the laboratory.
| Sensitivity | Specificity | Positive predictive value | Negative predictive value | Overall percent agreement | ||
|---|---|---|---|---|---|---|
| SARS-CoV-2 | GX | 96.6 (88.3–99.6) | 100.0 (93.7–100.0) | 100.0 (97.8–100.0) | 98.8 (95.7–99.9) | 99.1 (97.0–99.9) |
| TP | 96.6 (88.3–99.6) | 100.0 (98.0–100.0) | 100.0 (93.7–100.0) | 98.9 (96.0–99.9) | 99.2 (97.0–99.9) | |
| PC | 95.0 (85.9–98.9) | 99.4 (96.9–100.0) | 98.2 (90.6–100.0) | 98.4 (95.3–99.7) | 98.3 (95.8–99.5) | |
| Influenza A virus | GX | 100.0 (93.3–100.0) | 100.0 (98.0–100.0) | 100 (93.3–100.0) | 100.0 (98.0–100.0) | 100.0 (98.4–100.0) |
| PC | 100.0 (95.7–100.0) | 99.4 (96.9–100.0) | 98.8 (93.6–100.0) | 100.0 (98.1–100.0) | 99.6 (98.0–100.0) | |
| Influenza B virus | GX | 100.0 (47.8–100.0) | 100 (98.2–100.0) | 100.0 (47.8–100.0) | 100.0 (98.2–100.0) | 100.0 (98.2–100.0) |
| PC | 100.0 (47.8–100.0) | 98.6 (96.1–99.7) | 62.5 (24.5–91.5) | 100.0 (98.3–100.0) | 98.7 (96.2–99.7) | |
| InfluenzaA/B | TPa | 100.0 (96.0–100.0) | 100.0 (98.0–100.0) | 100.0 (97.9–100.0) | 100.0 (97.9–100.0) | 100.0 (98.6–100.0) |
| Respiratory syncytial virus | GX | 87.5 (71.0–96.5) | 100.0 (97.8–100.0) | 100.0 (87.7–100.0) | 97.6 (94.0–99.3) | 97.9 (94.8–99.4) |
| TP | 96.9 (83.8–99.9) | 100.0 (97.9–100.0) | 100.0 (88.8–100.0) | 99.4 (96.9–100.0) | 99.5 (97.4–100.0) | |
| PC | 100.0 (89.1–100.0) | 99.4 (96.9–100.0) | 97.0 (84.2–99.9) | 100.0 (97.9–100.0) | 99.5 (97.4–100.0) |
SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; GX, Xpert® Xpress SARS-CoV-2, Flu, RSV Kit; TP, TaqPath™ COVID-19, FluA/B, RSV Combo Kit; PC, PowerChek™ SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit II; FAO, Food and Agriculture Organization of United Nations recommended primers and probes developed by the Australian Center for Disease Prevention for the detection of M gene from avian influenza.
a
TP provides a combined influenza result for IAV and IBV as targets are combined in the same optical detection channel. Therefore, IAV and IBV results were combined for statistical tests.