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Experimental workflows used in this work. (a) the sample preparation steps for incubation of yeast lysates with xenon; (b) one-pot SPROX protocol to assay Xe-induced protein stability changes in proteins; (c) STEPP-LiP protocol to assay Xe-induced conformational changes in proteins; and (d) schematic representation of expected behavior of nonhit and hit proteins assayed in this work.
