Skip to main content
. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Nat Biomed Eng. 2022 Dec 29;7(2):94–109. doi: 10.1038/s41551-022-00964-5

Extended Data Fig. 3 |. Effect of iECM on metabolic activity and viability of rat cardiac endothelial cells, ROS scavenging, and thiol content.

Extended Data Fig. 3 |

Percent difference in reduction in alamarBlue activity of rat cardiac endothelial cells in response to hydrogen peroxide with and without iECM, relative to controls with no hydrogen peroxide (a, n=6 both groups, **p=0.006). Percent viability as measured by Calcein-AM staining of rat cardiac endothelial cells in response to hydrogen peroxide with and without iECM, relative to controls with no hydrogen peroxide (b, n=4 both groups, ***p<0.001). Concentration of hydrogen peroxide was monitored following incubation with either PBS or iECM, showing a continuing decrease with iECM (c, *p=0.01, **p=0.003, n=3 for both groups at 1 and 24 hrs, n=2 at 6 hrs). Thiol content was determined compared to N-acetylcysteine standard in iECM per mg of material (d, n=3). Data are mean ± SEM. All data are biological replicates and were evaluated with a two tailed unpaired t-test.