Figure 6:

iECM infusions are amenable to intracoronary infusion with a balloon infusion catheter. a, iECM complex viscosity is lower than its full ECM hydrogel counterpart, suggesting potential for catheter delivery. Note, error bars are too small to be visible for most measurements. N=3 all groups. b, Timeline of catheter feasibility study. c, Macroscopic short-axis view of a pig heart post-MI and iECM infusion. Infarct outlined in blue. d, Fluorescent image of iECM distribution and retention in a histological section from the same heart. Scale bar 200 μm. e,f, Representative confocal images of iECM (red) and endothelial cells (green) from iECM infused pigs. iECM lines the endothelial cells of infarcted myocardium, as similarly observed in the rat MI model. Lumen traced with dotted white lines; Scale bars 5 μm. g, iECM infusions showed a plateau effect with increasing infusion volumes, as suggested by quantified signal density in the infarct (n=2 for 1 and 10 mL, n=3 for 2 to 8 mL). Data are mean ± SEM. All data are biological replicates. b created with Biorender.com.