Table 1B.
Strains and Plasmids Used in This Study
| Cloning Strains or Plasmids | Genotype/Phenotype | Selection Marker | Reference |
|---|---|---|---|
| DH5α | E. coli cloning strain | — | 45 |
| RN4220 | S. aureus Restriction deficient cloning strain | — | 47 |
| pUC19 | Cloning vector | Amp | 44 |
| pUC19-Δe1 | 600bp flanking regions of e1 | Amp, Erm | This study |
| pUC19-Δe2 | 600bp flanking regions of e2 | Amp, Kan | This study |
| pUC19-USA300Δkq | 600bp flanking regions of seq sek | Amp, Kan | This study |
| pCL52.2 | E. coli S. aureus shuttle vector | Tet | 46 |
| pCL52.2-Δe1 | 600bp flanking regions of e1 | Tet, Erm | This study |
| pCL52.2-Δe2 | 600bp flanking regions of e2 | Tet, Kan | This study |
| pCL52.2- USA300Δkq | 600bp flanking regions of seq sek | Tet, Kan | This study |
| pRMC2 | Expression vector | Cam | 49 |
| pRMC2-e1 | Complementation of IHMA70 e1 enterotoxin cluster | Cam | This study |
| pRMC2-e2 | Complementation of IHMA70 e2 enterotoxin cluster | Cam | This study |
| pRMC2-kq | Complementation of USA300 sek seq enterotoxin cluster | Cam | This study |
| pRMC2-selX | Complementation of USA300 selX | Cam | This study |
Amp, ampicillin; Cam, chloramphenicol; Erm, erythromycin; Kan, kanamycin; Tet, tetracycline