Table 2.
Primers Used for the Knockout and Complementation of Enterotoxins in IHMA70 and USA300
| Primer | Target | Sequence |
|---|---|---|
| SEJ-REF | 600bp upstream of sej | AATAACTGCAGTACAGAACCAAAGGTAGAC |
| SEJ-RER | AGGTCGACAACAAGTAGATCTATACGG | |
| SER-REF | 600bp downstream of ser | CTGGTACCTGACTGGTGCTATG |
| SER-RER | TTTGAATTCTAACATGAATACACCTC | |
| SEK-REF | 600bp upstream of sek | TTTGACTGCAGTAAATTGGCTACTTACTC |
| SEK-RER | TCAGTCGACTCCTTGAGTATATTGGTTG | |
| SEQ-REF | 600bp downstream of seq | CGAGGTACCAGTACAAAGACCCACTC |
| SEQ-RER | TCAGTCGACTCCTTGAGTATATTGGTTG | |
| e1Comp-F | 150bp upstream of sej | CAACATCGGATCCTATTCTCATAGAATTTGTCTAATTAAGTGTACG |
| e1Comp-R | 150bp downstream of ser | GTCTCTCGAGGATGTTAAAGTATTTGAATTGACTAC |
| e2Comp-F | 150bp upstream of sek | TTACAACTCGAGACTCGGAAGATGATAAAACTAAAAGAGAC |
| e2Comp-R | 3ʹ end of seq | GGCGGGCTAGGATCCCCGAAAAATAATG |
| USA300K-F | 600bp downstream of sek | GCATTGGGAATTCGCCTTTATGATTAGTAAATAC |
| USA300K-R | CGGGTACCGCATGCCTACCC | |
| USA300Q-F | 600bp upstream of seq | ACTCTCAACGGATCCTCAAAT |
| USA300Q-R | GTACCACGTTTACACCTGCAGCTATC | |
| UCompKQ-F | 150bp upstream of seq | CCTCGAATTCGTGTACAAGATAAA |
| UCompKQ-R | 3ʹ end of sek | AGATCACCTCTGGTACCAAA |
| UCompX-F | 150bp upstream of selX | ACGAAAGGATCCAACGCATGACG |
| UCompX-R | 3ʹ end of selX | CATGCATTTAGCTGACTTCTGCAGTTG |