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. 2023 Apr 24;14:1123716. doi: 10.3389/fmicb.2023.1123716

Table 1.

Formulation, proximate composition (in dry form) and fatty acid profile of the control diet and the basal diet supplemented with a blend of bile salts (BSs) at a dietary inclusion of 0.06% (BS0.06%).

Ingredients (%) Control BS0.06%
Fishmeal super prime 7.50 7.50
Fishmeal 60 5.00 5.00
Fish protein concentrate 2.00 2.00
Feather meal hydrolysate 5.00 5.00
Porcine blood meal 3.00 3.00
Poultry meal 15.00 15.00
AminoPro NT70—C. glutamicum 4.00 4.00
Corn gluten meal 8.00 8.00
Soybean meal 48 12.00 12.00
Sunflower meal 5.00 5.00
Wheat meal 10.31 10.31
Whole peas 5.00 5.00
Pea starch (raw) 2.40 2.40
Fish oil 3.02 3.02
Soybean oil 2.35 2.35
Poultry fat 8.04 8.04
Vitamin and mineral premix 1.00 1.00
Vitamin C35 0.05 0.05
Vitamin E50 0.02 0.02
Betaine HCl 0.20 0.20
Choline chloride 60 0.10 0.10
Antioxidant 0.20 0.20
Sodium propionate 0.10 0.10
Monoammonium phosphate 0.35 0.35
L-Tryptophan 0.15 0.15
DL-Methionine 0.20 0.20
Bile salts mix - 0.06
Yttrium oxide 0.02 0.02
Proximate composition
Crude protein, % 44.1 ± 0.05 44.0 ± 0.08
Crude fat, % 18.1 ± 0.04 18.2 ± 0.05
Gross energy, MJ kg−1 21.4 ± 1.11 21.5 ± 1.20
Fatty acid profile (% of total fatty acids)*
Saturated fatty acids (SFAs) 27.19 ± 0.40 26.55 ± 0.06
Monounsaturated fatty acids (MUFAs) 36.61 ± 0.73 36.60 ± 0.22
n-6 Polyunsaturated fatty acids (n-6 PUFAs) 26.65 ± 0.06 27.11 ± 0.45
n-3 Polyunsaturated fatty acids (n-3 PUFAs) 9.55 ± 0.39 9.74 ± 0.09
Total PUFAs 36.20 ± 0.45 36.86 ± 0.36
*

Fatty acid profile of experimental diets is detailed in Supplementary Table 1. The proximate and fatty acid composition of diets were analyzed in duplicate, and values are represented as mean ± SD.