FIG. 7.

Southern blot analysis of DNA from other Borrelia species hybridized with a probe derived from the identified B. hermsii cp32 PF-144 locus. (A) Genomic DNA from B. parkeri (lanes 1 to 4), B. anserina (lanes 5 to 8), and B. burgdorferi (lanes 9 and 10). DNAs were either uncut (lanes 1, 5, and 9) or cut with EcoRI (lanes 2, 6, and 10), EcoRV (lanes 3 and 7), or HindIII (lanes 4 and 8) and separated by pulsed-field agarose gel electrophoresis. Uncut circular DNAs form a broad smear extending from the gel well under the electrophoresis conditions used in these experiments (54). (B) B. parkeri genomic DNA separated by two-dimensional agarose gel electrophoresis. Linear DNA is marked with an arrow. Molecular size markers (in kilobases) are indicated to the left of each panel.