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. 2023 Mar 28;20:41.

Table 4.

Summary of bisphenol-A release at different time points

Study n Groups Time 1 Time 2 Time 3 Time 4 Time 5 Time 6






Mean Variance Mean Variance Mean Variance Mean Variance Mean Variance Mean Variance
Kotykaetal., (2014) n=8 retainer material cut into squares 1-Prethermoformed and thermoformed Biocryl Essix 0 0 0 0 0 0 0 0
2-Prethermoformed Biocryl retainer 0 0 0 0 0 0 0 0
3-Thermoformed Biocryl retainer 760 846,400 0 0 0 0 0 0
4-Prethermoformed and thermoformed dentsply raintree Essix A 0 0 0 0 0 0 0 0
5-Unused and used Invisalign aligner 0 0 0 0 0 0 0 0
Raghavanetal., (2017) n=45 patients 1-Vacuum-formed retainers 0.01 0.0001 1203.36 127042.3 2384.2 3229712 203.96 7584.668
2-Hawley retainersheat cure 0.06 0.0016 0.91 0.6561 0.45 0.0064 0.67 1.9881
3-Hawley retainerschemical cure 0.09 0.0036 60.31 650.25 3.63 0.25 9.34 5.6169
El Idrissi Ietal., (2020) n=10 new aligners 110 new “SCHEU Dental Clear Aligner®”aligners 0 0 For up to 8 weeks
10 used aligners 2-10 used aligners from the same supplier after stay in the mouth of 15days 0 0 For up to 8 weeks
Katrasetal., (2021) n=37 1-Smile direct Club aligner in artificial gastric fluid on T0, T1, T2, T6, T10, T20 1.53 0.3844 2.82 0.3364 0.7 0.0196 3.11 0.7225 2.4 0.2116 2.02 0.7569
2-Smile direct Club aligner in artificial saliva on T0, T1, T2, T6, T10, T20 1.4 0.0441 3.02 0.81 4.02 0.16 3.72 0.4225 3.82 0.2116 5 5.5696
3-Essix ace aligner in artificial gastric fluid on T0, T1, T2, T6, T10, T20 1.53 3844 3.54 0.2704 3.13 0.0625 2.73 0.6889 2.65 0.0576 1.4 0.1089
4Essix ace aligner in artificial saliva on T0, T1, T2, T6, T10, T20 1.4 0.0441 3.26 6.9696 3.37 11.9716 4.5 11.8336 6.3 22.5625 6.18 13.9876
5-Invisalign aligner in artificial gastric fluid on T0, T1, T2, T6, T10, T20 1.53 0.3844 2.39 0.09 2.66 0.0324 1.99 0.0484 2.49 0.4096 1.9 1.4641
6-Invisalign aligner in artificial saliva on T0, T1, T2, T6, T10, T20 1.4 0.0441 3.53 3.4596 3.16 1.7956 3.49 1.7689 0.58 0.0049 0.51 0.1296
7-Smile direct Club aligner in ethanol on T0, T1, T2, T6, T10, T20 1.01 0.0625 1.75 0.04 - - - -0 1.9 0.0169 1.8 0.1444
8-Essix Ace aligner in ethanol on T0, T1, T10 and T20 1.01 0.0625 1.52 0.2916 - - - - 1.87 0.1024 0.47 0.0529
9-Invisalign aligner in ethanol on T0, T1, T10 and T20 1.01 0.0625 7.89 17.9776 - - - - 9.18 22.09 3.89 6.8644