Skip to main content
. Author manuscript; available in PMC: 2023 Jul 15.
Published in final edited form as: Biol Psychiatry. 2022 Nov 9;94(2):153–163. doi: 10.1016/j.biopsych.2022.11.005

Figure 4.

Figure 4.

Analysis of neuronal network activity and transcriptome in SCZ patient–derived iENs. (A) Representative well of patient iENs on multielectrode arrays. (B) Developmental time course of healthy and SCZ-patient iEN mean firing rate. (C) Developmental time course of healthy and SCZ-patient iEN synchrony index. (D) Representative raster plot of healthy and SCZ-patient line activity. Well and plate averages of firing rate (E), synchrony index (F), and network burst frequency (G) at 7WIV (N = 6, 3 patients per condition from 2 independent differentiations, n = 30 wells per condition). (H) Volcano plot of dysregulated gene expression in patients with SCZ with top 10 DEGs highlighted (black, 14,315 expressed with mean read counts > 5). (I) Significantly enriched gene ontology terms within SCZ iENs overlapping with isogenic lines. Volcano plot of top overlapping gene ontology terms; homophilic cell adhesion (J), glutamatergic synapse (K), and calcium ion binding (L) and heatmap of aggregated DEGs from these terms (M). (N) Venn diagram of overlapping DEGs from isogenic (FDR ˂ 0.1) and patient (FDR ˂ 0.3) lines showing shared direction. An enrichment of 2.11, p = .002, was observed. (O) Heat plot of significantly upregulated (FDR < 0.1) isogenic DEGs with shared direction in patient lines. (P) Heat plot of significantly downregulated (FDR ˂ 0.1) isogenic DEGs with shared direction in patient lines. *p < .05, **p < .01, ****p < .001. BP, biological process; CC, cellular component; DEG, differentially expressed gene; FDR, false discovery rate; iENs, induced excitatory neurons; MF, molecular function; SCZ, schizophrenia; WIV, week in vitro.