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. 2023 Mar 3;299(4):104578. doi: 10.1016/j.jbc.2023.104578

Figure 4.

Figure 4

Increased CCTα protein expression, dephosphorylation, and nuclear envelope localization in CEPT1-KO cells.A, lysates of U2OS and CEPT1-KO cells were immunoblotted for CCTα and the phosphosite-specific antibodies pS319 and pY359/pS362. B–D, CCTα protein expression (panel B) and phosphorylation of S319 (panel C) and Y359/S362 (panel D) were quantified from immunoblots. Results in panel B were normalized to OSBP, panels C and D were normalized to CCTα and then expressed relative to results with U2OS cells (mean and SD of three biological replicates). E, immunofluorescence confocal microscopy of CCTα in U2OS and CEPT1-KO cells (The scale bar represents 10 μm, images are representative of three independent experiments). The nucleus is stained with DAPI. F, lysates of CHPT1- and CEPT1-KO cells were immunoblotted for CCTα and CEPT1. CCT, CTP:phosphocholine cytidylyltransferase; CEPT1, choline/ethanolamine phosphotransferase 1; CHPT1, choline phosphotransferase 1; OSBP, oxysterol binding protein.