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. 2023 Mar 3;299(4):104578. doi: 10.1016/j.jbc.2023.104578

Figure 5.

Figure 5

CCTα localizes to the nucleoplasmic reticulum in CEPT1-KO cells.A, immunofluorescence confocal microscopy of U2OS and CEPT1-KO cells stained with antibodies against CCTα and PML. Arrows indicate patches of PML on the NE (bar 10 μm). B, immunofluorescence of U2OS and CEPT1-KO1 cells stained with antibodies against CCTα and LMNA/C. Arrows indicate localization of CCTα on NR structures (bar 10 μm). C, CHPT1-KO1 cells immunostained for CCTα and PML or PML and LMNA/C (The scale bar represents 10 μm). Images are representative of results from three independent experiments. CCT, CTP:phosphocholine cytidylyltransferase; CEPT1, choline/ethanolamine phosphotransferase 1; NE, nuclear envelope; PML, promyelocytic leukemia.