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. 2023 Mar 3;299(4):104578. doi: 10.1016/j.jbc.2023.104578

Figure 9.

Figure 9

CEPT1 KO increases nLDs enriched in CCTα.A, confocal immunofluorescence localization of PML and CCTα in U2OS, CEPT1-KO, and CHPT1-KO cells treated with 300 μM oleate for 24 h (The scale bar represents 10 μm). B, quantitation of total nuclear LDs (nLD) detected by BODIPY 493/503 staining. C, quantitation of PML-positive nuclear LDs (LAPS). D, quantitation of CCTα,positive nuclear LDs. Results in panels B, C, and D show the mean and SD from analysis of 15 to 50 fields of cells in three or four separate experiments (representative images are shown in panel A). Significance was determined by one-way ANOVA and Tukey’s multiple comparison tests. CCT, CTP:phosphocholine cytidylyltransferase; CEPT1, choline/ethanolamine phosphotransferase 1; CHPT1, choline phosphotransferase 1; LAPS, lipid-associated PML structure; LD, lipid droplet; PML, promyelocytic leukemia.