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. 2023 Apr 25;16:1161127. doi: 10.3389/fnmol.2023.1161127

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Copyright © 2023 Thorson, Wei, Johnson, Gabriel, Arshavsky and Pearring.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The number of rod photoreceptors expressing GFPf in the Nrl:CreERT2 mouse stabilizes 3 days post tamoxifen induction. (A) Representative retinal whole mounts from Nrl:CreERT2/GFPf stained with anti-GFP antibody showing GFP expression in the rod synapse at 2, 3, and 5 days post-injection (DPI). Scale Bars, 10 μm. (B) Bar graph plotting the number of GFP positive rods in a 2,500 μm² retinal area. Mean for each timepoint is displayed above the bar. Error bars represent S.D. A one-way ANOVA was preformed: ****p < 0.0001, ***p = 0.0003, ns p = 0.2172. (C) Representative retinal cross-sections from Nrl:CreERT2/GFPf mice at 2, 3, and 5 DPI stained with anti-GFP antibody and WGA. Nuclei are counterstained with Hoechst. Scale Bars, 10 μm.