Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 25;10:1135141. doi: 10.3389/fcvm.2023.1135141

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Yoshida, Uchida, Kodo, Ishizaki-Asami, Maeda, Katsumata, Yuasa, Fukuda, Kosaki, Watanabe, Nakagawa and Yamagishi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Functional analyses of TBX20. (A) Sequence chromatograms of TBX20 in the family. (B) The conservation of alignment of the variant in the coding regions between species. Changes in amino acids are highlighted in red. The position of T331 is well-conserved between species. (C) Structure of the human TBX20 protein. The position of the variant is indicated by an arrowhead. (D) Western blot analysis of the c-Myc-tagged wildtype TBX20 and the variant TBX20 (p.T331A) proteins. Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, is used as an internal control. (E) Fold increase in relative luciferase activity in HeLa cells transfected with green fluorescent protein as control, wildtype TBX20 or variant TBX20 (p.T331A) expression construct, and NPPA-Luc (N = 5). All values are expressed as the mean ± SD. Significance was assessed using one-way ANOVA, followed by Tukey's post hoc test. **, p < 0.01; N.S., not significant.