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. 2023 Apr 25;10:1135141. doi: 10.3389/fcvm.2023.1135141

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© 2023 Yoshida, Uchida, Kodo, Ishizaki-Asami, Maeda, Katsumata, Yuasa, Fukuda, Kosaki, Watanabe, Nakagawa and Yamagishi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Decreased expression of Pitx2 in TBX20 homozygous embryos. (A) Quantitative RT-PCR results show the significant decrease in Pitx2c expression in Tbx20^−/− embryos, compared with that in Tbx20^+/+ or Tbx20^+/− at embryonic day 9.5. Gapdh was used as internal control. Data were analyzed using one-way ANOVA, followed by Tukey's post hoc test. **, p < 0.01; N.S., not significant. (B) Whole-mount in situ hybridization with Pitx2 antisense riboprobe. Pitx2 expression dramatically decreases in the area of the cardiac inflows of Tbx20^−/− embryos (white arrows in the lower panels), compared to that of Tbx20^+/+ (black arrows in the upper panels) at embryonic day 9.5. 1st pa; first pharyngeal arch; la, left atrium; lv, left ventricle; oft, outflow tract; rv, right ventricle; v, ventricle. Scale bars, 500 µm.