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. 2023 Apr 25;14:1161674. doi: 10.3389/fmicb.2023.1161674

Table 2.

Overview of read numbers obtained for the 48 librairies and the merge value for the 12 samples.

Name Rep.* Raw reads Clean reads Identified reads Viral reads Coef. of var. %** Viral reads %***
2006-D 1
2
3
4
4,506,340
2,402,894
3,606,002
3,206,050
688,804
393,000
393,464
312,984
387,983
266,782
289,073
120,321
361,518
249,453
288,018
119,597
40
Merge 13,721,286 1,788,252 1,323,336 1,272,409 71
2006-G 1
2
3
4
9,541,300
11,217,902
2,966,010
6,423,356
2,260,746
1,530,728
356,374
598,924
1,488,848
1,274,720
209,670
340,585
990,656
1,261,356
208,750
335,904
88
Merge 30,148,568 4,743,756 3,699,942 3,167,343 67
2006-M 1
2
3
4
17,134
47,644,656
14,262,232
76,825,830
5,236
4,573,184
1,191,024
7,576,116
2,582
4,187,147
930,015
7,053,620
2,582
4,173,108
928,085
7,026,021
106
Merge 138,749,852 13,343,538 12,436,823 12,386,553 93
2006-G2 1
2
3
4
29,093,288
8,137,376
85,435,164
1,565,594
4,081,130
680,206
8,064,160
132,970
3,522,608
562,770
7,529,846
16,431
3,041,567
562,451
7,509,918
14,371
123
Merge 124,231,422 12,953,180 11,718,516 11,211,954 87
2007-P 1
2
3
4
5,774,206
9,458,542
8,181,372
3,455,326
92,5,076
919,580
862,832
338,988
464,453
620,486
676,385
162,431
410,478
611,586
673,825
158,685
50
Merge 26,869,446 3,044,114 2,149,086 2,061,661 68
2009-P 1
2
3
4
3,772,094
4,836,042
372,442
5,450,408
698,092
587,498
37,518
448,972
374,629
407,026
22,264
209,349
213,068
300,583
17,321
180,879
67
Merge 14,430,986 1,770,770 1,107,117 795,808 45
2012-K 1
2
3
4
14,176,218
9,356,070
3,554,214
36,261,558
4,587,968
2,572,568
450,022
2,545,060
1,060,613
2,110,438
197,616
2,086,422
558,029
1,684,264
137,633
2,038,462
82
Merge 63,348,060 10,150,928 5,871,134 4,810,896 47
2014-K2 1
2
3
4
1,856,764
3,872,212
52,270,136
736,618
558,526
588,342
3,846,026
263,830
256,651
324,504
3,371,485
20,151
98,446
213,169
3,300,670
2,553
109
Merge 58,735,730 5,256,534 4,041,606 3,686,030 70
2014-K1 1
2
3
4
12,176,666
16,887,440
3,298,316
3,114,878
2,336,528
1,544,840
298,646
239,354
1,514,188
1,115,033
162,226
128,259
984,878
779,709
150,098
118,964
87
Merge 35,477,300 4,418,862 3,153,740 2,248,319 51
2014-K 1
2
3
4
9,137,872
12,253,584
5,421,844
39,869,020
1,418,210
1,132,042
447,440
4,487,688
877,541
858,364
222,963
3,574,163
789,752
850,963
218,749
3,444,980
177
Merge 66,682,320 7,485,152 5,957,168 5,720,168 76
2016-B 1
2
3
4
10,936,716
68,870,930
39,108,178
21,645,010
2,801,174
8,652,778
3,875,918
2,476,722
1,762,383
7,858,150
3,124,556
2,031,273
1,440,351
7,735,805
3,042,426
2,012,830
81
Merge 140,560,834 17,795,292 15,290,037 14,708,048 83
2016-B2 1
2
3
4
24,749,188
48,291,662
715,534
31,200,266
5,210,512
13,195,394
104,526
3,267,994
3,080,758
11,603,668
76,346
2,112,003
2,350,567
9,160,827
75,782
2,029,485
117
Merge 104,956,650 21,772,204 17,266,476 14,007,068 64
Control 35,160 11,458 414 0
*

Data are provided for each replicate (rep.) and for the merge value of all four replicates for each sample (grey line),

**

Coefficient of variation is calculated as the ratio of the standard deviation σ to the mean μ , cv=σμ.

***

Percentage of viral reads calculated based on viral reads divided by clean reads, and multiplied by 100.