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Western blot reactivity of antigens from whole organisms of B. abortus strains RB51WboA, RB51, and 2308 with Bru38 (A) and goat serum hyperimmunized to RB51 antigens (B). Antigens of strains 2308, RB51WboA, and RB51 were separated by sodium dodecyl sulfate–12.5% polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane, and reacted with the indicated antibodies. Lanes labeled 1 and 2 contain antigens from two different colonies of strain RB51WboA.
