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. 2023 Apr 28;112(1-2):61–83. doi: 10.1007/s11103-023-01348-2

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© The Author(s) 2023

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Fig. 4 — EMSA of TRB1, TRB4 and TRB5 binding of radioactively-labelled oligonucleotides. (A) Schematic depiction of oligonucleotides employed. Telomeric, four repeats of plant telomeric DNA sequence; telo-box, 1.2 plant telomeric units flanked with non-telomeric DNA sequence; non-telomeric, oligonucleotide with non-telomeric DNA. (B) EMSA of TRB1, TRB4 and TRB5 proteins binding radioactively-labelled double-strand (ds) tetramers of telomeric sequence with unlabelled tetramers of non-telomeric oligonucleotides as competitor DNA. The concentration of unlabelled competitor increases from 1-, 20- to 100-fold the concentration of the labelled probe (as depicted by the triangle). Oligo*: protein ratio is 1:10. (C) EMSA of the same proteins with a radioactively-labelled ds telo-box oligonucleotides with unlabelled non-telomeric oligonucleotides as competitor DNA, performed as in B. (D) EMSA of the same proteins with a radioactively-labelled ds of non-telomeric oligonucleotides with unlabelled ds tetramers of telomeric sequence as competitor DNA, performed as in B