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. 2022 May 2;85(4):1498–1513. doi: 10.1007/s00248-022-02025-5

Fig. 1.

Fig. 1

Summary of experimental approach. (A) Highlights the marked bee assay and treatment/control conditions. Newly emerged bees (NEW’s) were collected from a climate controlled room and marked with a dot of paint on their thorax. The three treatments were fresh pollen, autoclaved pollen, and no pollen, all applied in social isolation in a climate controlled room. All treatment cages were provided autoclaved sucrose syrup and water ad libitum. Control colonies contained over 10,000 established (older) workers and were exposed to the pollination environment. (B) Illustration of tissue dissection and molecular analyses. The pylorus and ileum (P/I) were dissected, sequenced and analyzed as a unit