Fig. 3.

Throughout the alimentary tract, the presence of fungi (the mycobiome) or fungal associated factors was correlated with the structure of the bacterial microbiome. In our positive control, colony reared bees, there is a positive association of microbiota and mycobiota size, but considering the alimentary tract as a unit, fungal load decreases concurrent with increasing bacterial load (Rsq = 0.25, F = 11.4503, p = 0.002). This pattern shifted significantly when workers were detached from the active hive environment and provided various diet treatments. Values are from Fig. 2