Fig. 3. Bis-ANS binding and the importance of hydrophobic motifs for chaperone activity of Bri2 BRICHOS.

a Normalized Trp fluorescence for rh Bri2 BRICHOS T206W monomers and oligomers. b Amino acid sequences of loop motifs 1–3 mutants (boxed), see Fig. 1 for entire loop sequences. c Aggregation mass of 1.2 µmol L⁻¹ CS at 45 °C in the absence or presence of the different loop mutants at 1:1 (CS: BRICHOS) molar ratio. The aggregation mass are from three replicates. Data are presented as means ± standard deviations.