. 2023 May 8;14:2571. doi: 10.1038/s41467-023-37702-7

Table 1.

Transport activities of Mfsd2a mutants

Residue	Mutation	Transport activity	drMfsd2a site	DrMfsd2a lysolipid	drMfsd2a function
Tyr50	Ala²⁹	Reduced	Z_A	Lysolipid_1A	H-bond
Gln51	Leu¹⁹	Reduced	Z_A-B	Lysolipid_1A	H-bond
Phe59	Ala²⁹	Reduced	Chamber₁	Lysolipid_{1A, 1B}	Lipid tail interaction
Arg84	Ala/Lys/Glu¹⁹	Abolished	Z_A	Lysolipid_{1A, 1B}	Salt-bridge
Asp87	Ala²⁹	Abolished	Z_A	Lysolipid_1A	Salt-bridge
His156	Ala²⁹	Abolished	Z_B	Lysolipid_2B	H-bond
Ser160	Leu^26,29	Reduced	Z_B	Lysolipid_2B	H-bond
Arg180	Ala²⁹	Abolished	Z_B	Lysolipid_{1B, 2B}	Salt-bridge
Met181	Ala/Phe¹⁹	Reduced	Chamber₂	Lysolipid_2B	Lipid tail interaction
Thr182	Phe/Met²⁹	Reduced	Chamber₃	Lysolipid_3c	Lipid tail interaction
Glu184	Ala²⁹	Reduced	Z_A-B	Lysolipid_{1A, 1B}	H-bond
Thr188	Phe²⁹	Reduced	Chamber_1-2	Lysolipid_{1A, 1B, 2B}	Lipid tail interaction
Phe298	Ala²⁹	Reduced	Chamber₁	Lysolipid_{1A, 1B}	Lipid tail interaction
Leu308	Trp¹⁹	Reduced	Chamber₁	Lysolipid_{1A, 1B}	Lipid tail interaction
Phe312	Ala/Tyr¹⁹	Reduced	Chamber₁	Lysolipid_{1A, 1B}	Lipid tail interaction
Ile333	Ala¹⁹	Reduced	Chamber₁	Lysolipid_{1A, 1B}	Lipid tail interaction
Ile341	Trp¹⁹	Abolished	Chamber_2-3	Lysolipid_{2B, 3C}	Lipid tail interaction
Gln345	Trp¹⁹	Reduced	Z_C	Lysolipid_3C	H-bond
Ala388	Trp¹⁹	Reduced	Chamber₁	Lysolipid_{1A, 1B}	Lipid tail interaction
Met334	Ala¹⁹	Reduced	Chamber_1-2	Lysolipid_{1A, 1B, 2B}	Lipid tail interaction
Phe396	Ala/Ile/Trp¹⁹	Reduced	Chamber_1-2	Lysolipid_{1A, 1B, 2B}	Gating, Lipid tail interaction
Trp400	Ala/Phe¹⁹	Reduced	Chamber_1-3	Lysolipid_1-3	Gating, Lipid tail interaction
Glu421	Arg³³	Reduced	Z_A	Lysolipid_{1B, 2B, 3C}	Salt-bridge
Thr432	Ala²⁹	Increased	Z_A-B	Lysolipid_1A	H-bond
Lys433	Ala²⁹, Gln¹⁹	Abolished/Reduced	Z_A	Lysolipid_1A	Salt-bridge