Figure 3.

Selective activation of DMN cholinergic neurons reduces the histological severity of acute pancreatitis (A–I) Representative images of pancreatic tissue stained with H&E at 4x (left panels), 10x (middle panels), and 20x (right panels) from (A–C) non-caerulein-injected and non-stimulated controls, (D–F) caerulein-injected and yellow-light-stimulated, and (G–I)) caerulein-injected and blue light-stimulated mice. Bar = 200 μm. Histological scoring for (J) hemorrhage and fat necrosis, (K) acinar necrosis, (L) inflammation and perivascular infiltration, (M) edema, and (N) total severity. Data are represented as individual mouse data points with mean ± SEM. One way ANOVA with Kruskal-Wallis, *P P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, n = 9-11, ns, not significant.