Skip to main content
. 2023 Apr 25;17:1167047. doi: 10.3389/fnins.2023.1167047

Figure 5.

Figure 5

Reducing Pol III expression in OLs impairs myelination. (A) Representative immunofluorescence images (from three independent experiments per timepoint with similar results) of Shi/Shi organotypic slices transplanted with siRNA-treated OPCs (Ctrl siRNA, siPolr3a, siPolr3b, siPolr1c) showing Calbindin (magenta) and MBP (green) collected at 3 weeks or 6 weeks. Scale bar, 300 μm (10X), 150 μm (20X). (B,C) Quantitative analysis of organotypic shiverer slice culture assay, with % MBP+ area/Calbindin+ area depicted as mean ± SEM from three independent experiments (each with 2 technical replicates), determined from 10X objective at (B) 3- and (C) 6-week timepoints. **p < 0.01, ****p < 0.0001. (D) Immunofluorescence images of Shi/Shi organotypic slices transplanted with siRNA-treated OPCs (Ctrl siRNA, siPolr3a) in an axon-rich cross-section. Collected at 6 weeks. Scale bar, 300 μm (10X), 150 μm (20X). (E) Representative immunofluorescence images (from three independent experiments with similar results) of siRNA-treated OPCs cultured with nanofibers showing MBP (green). 20X objective, scale bar, 20 μm. (F) Quantification of MBP+ sheath length depicted as mean ± SEM from three independent experiments (each with 2 technical replicates). ****p < 0.0001, unpaired, two-sided Student’s t-test.