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. 2023 May 8;13:7470. doi: 10.1038/s41598-023-33822-8

Figure 8.

Figure 8

Cytotoxicity of endolysin (fusion- LysVTh2) to human epithelial cells. AB183 was grown overnight in Leibovitz medium and used to infect cells for 1 h before adding 2 mg/ml of lysAB-vT2-fusion to relevant samples and incubating for a further 23 h. LDH cytotoxicity assay was then performed using the Invitrogen CyQUANT kit. (A) Recovery and cytotoxicity of lysAB- vT2-fusion treatment of T24 human epithelial cells infected by A. baumanni AB183. (B) Cytotoxicity after recovery of T24 human epithelial cells from AB183 infection after treatment by simultaneous lysAB- vT2 fusion endolysin and antimicrobials; CuCl2 (1.62 mM) and colistin sulphate (0.5 µg/ml).