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. 2023 Apr 3;55(4):779–793. doi: 10.1038/s12276-023-00961-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Gene Ontology (GO) analysis of the cellular component subcategories positively regulated by SAMHD1 overexpression. b, c Gene set enrichment analysis (GSEA) related to cytoskeletal changes for cell migration in the SAMHD1 high expression group. ***p < 0.001. d, e Immunofluorescence (IF) with anti-paxillin antibodies and 4′,6-diamidino-2-phenylindole (DAPI) staining of SAMHD1 knockdown SN12C cells or SAMHD1 overexpressed Caki-1 cells. Scale bar, 20 μm. f mCherry-cortactin was transfected as a control, and SAMHD1 was knocked down in SN12C cells for lamellipodium observation (left). Scale bar, 20 μm. Graphs present the lamellipodium area in each cell (right) (n = 20 cells). Data are presented as the mean ± SEM. ***p < 0.001. g SAMHD1-overexpressing Caki-1 cells and control cells were transfected with mCherry-cortactin for lamellipodium observation (left). Scale bar, 10 μm. Graphs present the lamellipodium area in each cell (right) (n = 20 cells). Data are presented as the mean ± SEM. ***p < 0.001.