Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 3;55(4):779–793. doi: 10.1038/s12276-023-00961-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a, b GSEA analysis of SAMHD1 mRNA levels and endocytosis-related signaling pathways. c ACHN cells were transfected with control or siSAMHD1 and immunolabeled for EEA1. The nuclei were stained with DAPI. Scale bar, 20 μm. Graphs present the number of EEA1-expressing vesicles per single cell (n = 55 cells). Data are presented as the mean ± SEM. The experiments were independently performed three times. ***p < 0.001. d SAMHD1-overexpressing Caki-1 cells were immunolabeled for EEA1. The number of EEA1-expressing vesicles per single cell is shown (n = 55 cells). Scale bar, 20 μm. Data are presented as the mean ± SEM. The data shown are representative of three independent experiments. ***p < 0.001. e SAMHD1 colocalized with cortactin in the cytoplasmic circular-shaped organelles and on the lamellipodia. f Exogenous Flag-tagged SAMHD1 was immunoprecipitated with magnetic Flag beads from Flag-SAMHD1-overexpressing Caki-1 cells. g Endogenous SAMHD1 was immunoprecipitated from SAMHD1-knockdown SN12C cells. Anti-cortactin antibodies were used to examine SAMHD1 and cortactin binding. IgGs were incubated with cell lysates as negative controls. h Endogenous cortactin was immunoprecipitated from SAMHD1-downregulated ACHN cells. Anti-SAMHD1 antibody was used to examine SAMHD1 and cortactin binding. IgG was incubated with cell lysates as a negative control. i, j Phosphorylated cortactin (Y421) and total cortactin levels were evaluated using western blotting in SAMHD1-knockdown ACHN and SN12C cells or SAMHD1-overexpressed Caki-1 and Caki-2 cells. k IHC analysis of SAMHD1 and phosphorylated cortactin (Y421) in ccRCC patient tissues. Scale bar, 50 μm. l Areas with positive IHC staining were measured and are shown on the graph. Data are presented as the mean ± SD. ***p < 0.001; **p < 0.01. m Correlation between SAMHD1 and phosphorylated cortactin (Y421) expression from IHC analysis of ccRCC patient tissues.