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Time-dependent levels of TNF-α, IL-6, and IL-10 in plasma during stimulation of whole blood with PepG (A) or LTA (B). The whole blood was added 10 μg of PepG or 100 μg of LTA per ml of blood, and the blood was incubated at 37°C for 24 h. After the indicated periods of time, plasma was isolated and analyzed for TNF-α, IL-6, and IL-10 by EIA. Cytokine values are normalized to the highest value for each cytokine in 1 representative experiment of 10 performed.
