FIG. 3.

Expression of mRNA for TNF-α, IL-6, IL-10, and β-actin in various leukocyte populations 6 h after incubation of whole blood in the absence (lanes 1 to 4) or presence (lanes 5 to 8) of PepG, LTA (lanes 9 to 12), or LPS (lanes 13 to 16) at 37°C. CD14⁺, CD19⁺, CD2⁺, and CD15⁺ cells were isolated by immunomagnetic separation. mRNAs from these cells were isolated by oligo(dT)₂₅-coated magnetic beads, reverse transcribed, and analyzed for transcripts encoding TNF-α (panel A, 443 bp), IL-6 (panel B, 628 bp), IL-10 (panel C, 328 bp) and β-actin (panel D, 660 bp) by PCR. Note that by mistake, no RT product was added to the sample in lane 2 (panel B). Unstim., unstimulated.