TABLE 1.
Effect of pretreatment of whole blood with anti-CD14 MAb 18D11 on the ability of PepG, LTA, or LPS to induce TNF-α release
| Bacterial product (concn) | Concn of TNF-α released (pg/ml)a
|
||
|---|---|---|---|
| Without 18D11 | With 18D11 | With control antibody | |
| PepG (10 μg/ml) | 6,593 ± 1,350 | 9,226 ± 1,291 | 9,558 ± 1,240 |
| LTA (30 μg/ml) | 2,506 ± 220 | 1,188 ± 269b | 2,421 ± 314 |
| LPS (10 ng/ml) | 11,140 ± 635 | 3,533 ± 636b | 10,900 ± 778 |
a
Values are means ± SEM of six experiments. MAb 18D11 and control antibody were added at 5 μg/ml.
b
Significantly lower (P < 0.05) than the value for the untreated control group as calculated by analysis of variance with Tukey post hoc assessment.