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. 2023 Apr 30;35(2):176–190. doi: 10.21147/j.issn.1000-9604.2023.02.07

Figure 1.

Figure 1

A-GSP induces Fe2+ accumulation, GPX4 inactivation and lipid peroxide increase in OSCC cells. (A-C) Changes in intracellular Fe2+ content (A), GSH content (B) and intracellular MDA content (C) after 72 h of A-GSP treatment in SCC15 and SCC25 cells; (D) Content of intracellular lipid peroxides in each group was observed under a confocal microscope (Scale bars are 20 μm and 5 μm, respectively); (E) Statistical analysis of Liperfluo experimental results; (F) Flow software detected the ROS generation in each group of cells; (G) Statistical results of ROS fluorescence intensity; (H) Fold changes of proteins encoded by ferroptosis-related genes after 72 h of A-GSP treatment in OSCC cells. A-GSP, aqueous-soluble sporoderm-removed G. lucidum spore powder; OSCC, oral squamous cell carcinoma; GSH, glutathione; MDA, malondialdehyde; ROS, reactive oxygen species. **, P<0.01; ***, P<0.001.