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. 2023 Apr 30;35(2):176–190. doi: 10.21147/j.issn.1000-9604.2023.02.07

Figure 3.

Figure 3

A-GSP-induced reduction in OSCC cells viability can be rescued by ferroptosis inhibitors. (A) Cells were treated with a mixture of ferrostatin-1 and A-GSP at concentrations of 10 μmol/L and 1,000 μg/mL, respectively, for 72 h (All related experiments with the addition of ferrostatin-1 in this figure have the same treatment conditions). Rescue of cell viability was detected; (B) Cells were treated with a mixture of the ferroptosis inhibitor Liproxstatin-1 and A-GSP at concentrations of 15 μmol/L and 1,000 μg/mL, respectively, for 72 h. The recovery of cell viability was detected; (C) Ferrostatin-1 reversed the loss of self-cloning ability of oral cancer cells; (D) Statistical results of clone formation experiments; (E) Changes in MitoTracker Green-labeled mitochondria after adding ferrostatin-1 to the drug (Scale bars are 20 μm and 5 μm, respectively); (F) Statistical analysis results of MitoTracker Green experiments. A-GSP, aqueous-soluble sporoderm-removed G. lucidum spore powder; OSCC, oral squamous cell carcinoma. ***, P<0.001.