Skip to main content
. 2023 Apr 30;35(2):140–162. doi: 10.21147/j.issn.1000-9604.2023.02.05

Figure 4.

Figure 4

rs8400 A allele increases ALKBH5 levels and promotes neuroblastoma cancer progression. (A) In adrenal tissues, individuals with rs8400 AA genotype had statistically higher levels of ALKBH5 mRNA than those with rs8400 GG or GA genotype; (B) Immunohistochemistry results show that tissue samples with the rs8400 A allele have higher ALKBH5 expression relative to rs8400 G allele (Magnification 10×); (C,D) Luciferase activity was significantly reduced in SK-N-SH (C), SK-N-BE(2) (D) cells with the rs8400 G allele construct compared with the construct with the rs8400 A allele; (E) Overexpression of rs8400 A significantly promoted neuroblastoma cell proliferation compared to overexpression of rs8400 G or the control vector; (F) Flow cytometry showed a higher proportion of cells in G2/M phase in neuroblastoma cells with rs8400 A allele relative to rs8400 G allele; (G) Colony formation experiments further demonstrated that rs8400 A-overexpressing cells had the most colonies; (H) Wound closure ability of stably overexpressed rs8400 A allele was increased compared with stably overexpressed rs8400 G allele or control vector or blank group without any treatment (Magnification 10×); (I) Stable overexpression of rs8400 A also significantly promoted the migration and invasion abilities of SK-N-AS cells compared with stably overexpressed rs8400G or control vector or blank group (Magnification 10×). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001.