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Secondary structure energies of ALKBH5 3’UTR with different sequence lengths. To avoid sequence length-dependent effects, a nucleotide sequence around miR-186-3p seed binding site and SNP site was extended to a length of 40−52 nt toward the 5’ end. The final target sequence was determined to be 52 nt. SNP, single-nucleotide polymorphism; nt, nucleotide.
