Figure S3.

Construction of ALKBH5 knockdown neuroblastoma cells. (A) Results of RT-qPCR analysis showed that ALKBH5 mRNA expression was significantly reduced in ALKBH5 knockdown neuroblastoma cell lines compared with the control group; (B) Western blotting data showed that ALKBH5 knockdown neuroblastoma cell lines had significantly reduced ALKBH5 protein expression; (C) EdU analysis also showed that proliferation of neuroblastoma cell lines with knockdown of ALKBH5 expression was inhibited. RT-qPCR, reverse transcription-quantitative real-time polymerase chain reaction. ***, P<0.001; ****, P<0.0001.