Fig6. HLM006474 attenuates TGF-β1-induced fibroblast differentiation.
A. HLF cells were treated with TGF-β1 for 48 hour, and then cells were post-treated with HLM006474 for an additional 24 hour. FN and α-SMA were examined by immunofluorescence saining. B. Immunoblotting analysis of HLM006474-treated TGF-β1-induced lung myofibroblast cells. C. Immunoblotting analysis of HLM006474-treated IPF-LF cells. Immunoblots are representative of three independent experiments. D. IPF-LF cell migration was measured by a cell scratch assay. n=6, * P<0.01, compared to DMSO-treated cells.