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The six nucleotides in the forward and reverse primers recognized by the listed restriction enzymes are highlighted in bold.
| Primers | ||||
|---|---|---|---|---|
| Construct | Residues | Forward | Reverse | Molecular weight (Da) |
| GtfB | 861 (191–1051) | GGGGCCATGGGGGATGAAACTGGCGCTTATACT (NcoI) | GAACTCGAGTTTATTATCTGAAATATTAAA (XhoI) | 96330 |
| GtfD | 854 (248–1091) | GCTCGCTAGCATTGATAACTATGTCACAGCTGATTC (NheI) | GCTCCTCGAGGTTAGTCATCTGTTTTGGCAGAT (XhoI) | 95663 |
