TABLE 1.
Hydrogen peroxide sensitivity and production by various bacterial pathogens
| Species | MIC (mM)a | MBC (mM)b | H2O2 generated (mM)c | Source or referenced |
|---|---|---|---|---|
| Gram-negative | ||||
| H. influenzae Rd | 0.4 | 0.5 | <0.1 | 26 |
| H. influenzae Eagan | 0.4 | 0.5 | <0.1 | 26 |
| N. meningitidis MC58C3 | 0.4 | 5 | <0.1 | 32 |
| M. catarrhalis Bc1 | 1.1 | 160 | <0.1 | Clinical isolate |
| E. coli RS218 | ND | 15 | <0.1 | 1 |
| S. enterica serovar Typhimurium LT2 | ND | 20 | <0.1 | Collection of K. Sanderson |
| K. pneumoniae Kp1 | ND | 20 | <0.1 | Clinical isolate |
| P. aeruginosa PA01 | ND | 60 | <0.1 | ATCC 15692 |
| Gram-positive | ||||
| S. pyogenes P87 | ND | 40 | <0.1 | Clinical isolate |
| S. agalactiae P60 | ND | 80 | <0.1 | Clinical isolate |
| S. equisimilis P107 | ND | 20 | <0.1 | Clinical isolate |
| E. faecium P119 | ND | 80 | <0.1 | Clinical isolate |
| S. aureus A1 | ND | 10 | <0.1 | Clinical isolate |
| S. pneumoniae strains | ||||
| P394 (type 4) | 1.6 | 80 | TIGR genome strain | |
| D39 (type 2) | 1.2 | 80 | 0.44 ± 0.08 | 4 |
| P383 (type 6B) | ND | ND | 0.53 ± 0.08 | 22 |
| P384 (type 6A) | ND | ND | 0.71 ± 0.13 | 22 |
| P878 D39 (spxB::TnphoA) | 1.6 | 80 | <0.1 | 38 |
| P62 (type 9V opaque variant) | ND | ND | <0.1 | 22 |
| P64 (type 9V transparent variant) | ND | ND | 0.43 ± 0.13 | 22 |
a
The MIC was determined as the minimum concentration of H2O2 necessary to prevent turbid growth of a 1-in-50 inoculum of a stationary-phase culture following overnight incubation at 37°C. ND, not determined.
b
The MBC was determined as the minimum concentration of H2O2 necessary for >99.9% killing of washed, log-phase cells in BHI medium after 30 min at 37°C.
c
H2O2 concentration present in culture supernatants after incubating approximately 5 × 107 washed, log-phase cells for 1 h in BHI medium at 37°C.
d
TIGR, The Institute for Genome Research.