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. 2023 May 6;61(1):768–778. doi: 10.1080/13880209.2023.2207592

Figure 2.

Figure 2.

Angelica sinensis polysaccharides rescued perivascular mesenchymal progenitor growth inhibition via anti-senescence and anti-apoptosis effects. (A) Perivascular mesenchymal progenitors were cultured in 35 mm2 dishes in different groups. The viable cells were counted by Trypan blue staining (***p < 0.001 vs. control group; #p < 0.05 vs. 5-fluorouracil (5-FU) group, n = 3). (B) A cell counting kit (CCK)-8 assay was performed to detect perivascular mesenchymal progenitor growth (***p < 0.001 **p < 0.01 vs. control group; ##p < 0.01 #P < 0.05 vs. 5-FU group, n = 3). (C) The representative images of senescent perivascular mesenchymal progenitors are presented by senescence-associated β-galactosidase (SA-β-gal) staining. Senescent cells are blue-green stained (scale bar = 50 μm, n = 3). (D) The histogram of SA-β-gal staining positive rate of perivascular mesenchymal progenitors (***p < 0.001 vs. control group; #p < 0.05 vs. 5-FU group). (E) Annexin V-FITC/PI double staining was employed to detect cell apoptosis by flow cytometry. LL represented normal living cells, LR and UR represented early and late apoptotic cells, respectively. (F) The histogram of apoptosis rate of perivascular mesenchymal (***p < 0.001 vs. control group; ##p < 0.01 vs. 5-FU group).