Table 2.
A detailed list of primer sequences, species, gene bank numbers and polymerase chain reaction (PCR) product lengths used in real-time reverse transcription polymerase chain reaction (RT-PCR).
| Gene | Direction | Primer sequence (5′→3′) | GeneBank accession number |
PCR product size (bp) |
|---|---|---|---|---|
| Runx2 | Forward | AACAGCAGCAGCAGCAGCAG | NM_001271631.1 | 192 |
| Reverse | GCACCGAGCACAGGAAGTTGG | |||
| Osterix | Forward | GCGGCAAGGTGTATGGCAAGG | XM_006520519.5 | 176 |
| Reverse | GCAGAGCAGGCAGGTGAACTTC | |||
| OPN | Forward | AAGAGCGGTGAGTCTAAGGAGTCC | NM_001204203.1 | 91 |
| Reverse | TGCCCTTTCCGTTGTTGTCCTG | |||
| Ang-1 | Forward | TAACCGGATTCAACATGGGCA | NM_001286062.1 | 101 |
| Reverse | GAGCGTTGGTGTTGTACTGC | |||
| CXCL12 | Forward | TGACGGTAAACCAGTCAGCC | NM_001012477.2 | 129 |
| Reverse | CGTGCAACAATCTGAAGGGC | |||
| ICAM1 | Forward | CGGAGGATCACAAACGAAGC | NM_010493.3 | 149 |
| Reverse | CTCTTGCCAGGTCCAGTTCC | |||
| VCAM1 | Forward | GTTGTTCTGACGTGTGCTGC | NM_011693.3 | 129 |
| Reverse | CACAGAGCTCAACACAAGCG | |||
| VLA4 | Forward | TGTCCTACTGGTCCCGACAT | NM_010578.2 | 87 |
| Reverse | CCAAATCAGCAGCAAGGCAA | |||
| LFA1 | Forward | GAAGCTGAGCAGCCTTGTCT | NM_001253873.1 | 102 |
| Reverse | CTTGGAGAGTTCCACGGTCC | |||
| SCF | Forward | CGGGATGGATGTTTTGCCTA | XM_006513315.4 | 188 |
| Reverse | TCTTCGGTGCGTTTTCTTCC | |||
| MPL | Forward | GCCCAGATGGACTACCGAAG | NM_005373.3 | 110 |
| Reverse | TTGGCAATGTGGGTGGTACA | |||
| TPO | Forward | CTGGTTATGGCCTGCACAGA | XM_024453093.2 | 110 |
| Reverse | TTGCTTTCCTCCAAGACGCT | |||
| Tie-2 | Forward | CGACTGTAGCCGTCCTTGTA | XM_006502930.4 | 118 |
| Reverse | CGGCTCACAAGCTTTCTCAC | |||
| Notch | Forward | GCTCCGAGGAGATCAACGAG | NM_008714.3 | 268 |
| Reverse | TTGACATCACCCTCACACCG | |||
| Jagged-1 | Forward | CCATGCAGAACGTGAATGGAG | NM_013822.5 | 139 |
| Reverse | GTGACGCGGGACTGATACTC |