Fig. 5 |. Sex-specific LMNA ASO effect in LMNA^G/G mice.

One-month-old LMNA^G/G mice (n = 12 per group) were treated with control scrambled ASO SCR760 (50 mg kg⁻¹) or LMNA ASO L-B143 (17 mg kg⁻¹ and 50 mg kg⁻¹) for up to 10 months (Methods). a, Kaplan–Meier survival plots of the entire treatment group (male+female). b, Total body weights of LMNA^G/G mice treated with control scrambled ASO SCR760 (50 mg kg⁻¹) or LMNA ASO L-B143 (17 mg kg⁻¹ and 50 mg kg⁻¹) for up to 10 months. Mean values are represented, and error bars indicate s.d. c, d, Kaplan–Meier survival plots based on the sex showing a significant improvement in male lifespan (n = 12) of LMNA ASO L-B143-treated mice compared to females (n = 12). e, f, Protein detection. Total protein extract from pooled aortas treated with PBS, control scrambled ASO SCR760 or LMNA ASO L-B143 (3.5-month time point) and probed using human-specific anti-lamin A/C antibody (JOL2, ab40567). The data were normalized to housekeeping mouse GapDH gene (ab8245). e, Normalized aorta protein data treatment group (males+females) and (f) sex-specific analysis. SCR ASO, scrambled ASO760.