Table 3. Summary of bioassay data for synthetic ponericins.

IC₅₀, concentrations that caused 50% inhibition of H. contortus larval development; PD₅₀, median paralytic dose, and LD₅₀, median lethal dose for insecticidal effects on L. cuprina; MIC, minimum concentration for inhibition of bacterial growth; CC₅₀, concentration required to induce 50% death of HEK293 cells; HC₅₀, concentrations required to lyse 50% of human red blood cells; EC₅₀, effective concentration for activation of F11 cells in a FLIPR assay. All errors are SEM. Active peptides are shaded.

Peptide	Subfamily	Anthelmintic (IC50 μM)	Insecticidal (nmol g−1)		Antimicrobial (lowest MIC, μM)	Cytotoxicity (CC50 μM)	Hemolysis (HC50 μM)	DRG assay	FLIPR (EC50 μM)
			(PD50 1 h)	(LD50 24 h)					F11	HEK293
Na1b	w	2.8 ± 0.3	25.8 ± 13.9	>100	0.18–2.9a,b	4.5–5.9	39.9–61.5	Strong	0.99 ± 0.01	0.89 ± 0.05
Nc1a	w	5.1 ± 0.9	31.4 ± 6.3	32.6 ± 11.3	1.48–5.9a,b,c	12.6–13.5	28.6–48.2	Strong	1.35 ± 0.01	6.88 ± 1.98
Nc2a	L	23.2 ± 8.9	38.1 ± 18.5	>100	5.9 – 11.8b	48.2–56.9	>250	Weak	39.4 ± 10.7	86.9 ± 12.1
Nc3a	G	5.6 ± 1.3	0.5 ± 0.03	3.5 ±0.8	l.48–2.9a,b	5.2–6.8	>100	Strong	22.9 ± 1.95	57.9 ± 5.87
Nc3b	G	37.8 ± 7.4	26.4 ± 5.9	>100	>40	>300	>300	Inactive	>100	>100

^aAcinetobacter baumannii

^bBacillus subtilis

^cStaphylococcus aureus