Figure 2.

Ovarian hormones in a natural pregnancy in mice. P4 and E2 were measured in mouse serum from gestational day (D) 0 to D4. P4 levels begin to rise on D2 (0000 h), whereas serum E2 levels remain similar throughout early pregnancy. Each diamond represents data from one mouse. Mean ± SEM displayed in red. *P < 0.05. For serum hormone levels, the unpaired two-tailed Student’s t-test was performed with Welch’s correction. P4: Progesterone; E2: Estrogen. Three to seven mice were evaluated for serum hormones at each time point.